Mechanical control of mitotic progression in single animal cells Biology Diagrams Keywords: adhesion, integrin, cell cycle, mitosis, G2 phase. 1. Integrins. Cell adhesion to the ECM is mainly mediated by the integrin family of receptors, which sense the chemical and mechanical properties of the extracellular microenvironment and generate responses regulating many cellular functions, including cell proliferation [1,2]. These
Most adherent animal cells undergo drastic morphological changes upon entry into mitosis, beginning with the disassembly of cell adhesion to the extracellular matrix (ECM) and progressing with the During mitosis, cells become rounded and lose attachment to the substrate. Marchesi et al. show that DEPDC1B, a cell-cycle-regulated protein, binds to the focal-adhesion-associated receptor PTPRF, thus inhibiting RhoA activation and leading to dismantling of focal adhesions upon mitotic entry. DEPDC1B thus links mitotic progression to de-adhesion.
Reticular adhesions are a distinct class of cell Biology Diagrams
Initial biochemical studies investigating the link between cell cycle and adhesion remodeling focused on mapping changes in phosphorylation of focal adhesion signaling layer proteins FAK, p130Cas and paxillin during mitosis [19,20]. How these changes were linked to cell cycle progression however was unclear. The circuitry is relevant in whole organisms, as shown by the control exerted by the DEPDC1B/RhoA/PTPRF axis on mitotic dynamics during zebrafish development. Our results uncover an adhesion-dependent signaling mechanism that coordinates adhesion events with the control of cell-cycle progression. During mitosis, some interphase functions are retained, but the architecture of the cytoskeleton changes dramatically, and there is a need to generate a mitotic spindle for chromosome segregation. adhesion signalling and cell proliferation, particularly in the contexts of normal growth control and aberrant tumour progression. As the
Adhesion to the extracellular matrix persists during mitosis in most cell types. However, while classical adhesion complexes, such as focal adhesions, do and must disassemble to enable mitotic Therefore, ฮฑVฮฒ5-positive reticular adhesion complexes are essential for the normal progression of mitosis in cultured cells. 3. Future directions (a). Regulation of cell-cycle-dependent adhesion transitions. Having established a novel fundamental link between cell-cycle progression and cell-ECM adhesion, a number of questions are outstanding.
